Yocell Biotechnology (Qingdao) Co., LTD

In the field of life sciences, the research on exosomes has gradually become a hot topic, and its influence on people's lives is also multi-faceted. From the early detection of cancer to immune regulation, the application of exosomes has permeated all aspects of medical and health care. For instance, studies have found that exosomes can carry specific miRNAs for non-invasive diagnosis of cancer; they can also help treat inflammatory diseases by regulating the immune system.

The separation and purification of proteins is a complex process. Usually, different purification strategies are adopted based on the varying properties of proteins such as size, solubility, and charge. In this article, we will introduce the basic principles and practical applications of protein purification techniques.

In recent years, with the rapid development of the biopharmaceutical industry, antibody drugs have become an important means of treating major diseases such as cancer and autoimmune diseases. In the production process of antibody drugs, purification is one of the key steps, and tangential flow filtration, as an efficient separation technology, plays a crucial role in the formulation of antibody drugs.

Biopharmaceuticals are medicines prepared using biomanufacturing techniques. Compared with traditional medicines, biopharmaceuticals are characterized by being derived from biological products, highly specific, safer and more effective. Currently, biopharmaceuticals are becoming a focal area of development in the pharmaceutical industry, and injectable biologics have accounted for almost 90% of the entire biopharmaceutical market. The main method used in the manufacturing process of biopharmaceuticals is fermentation technology. Fermentation technology utilizes the metabolic capacity of microorganisms for the conversion and production of organic and inorganic substances. The microorganisms used in biopharmaceuticals are cell culture toxin-producing bacteria, protein-expressing bacteria, and cell culture viruses.

Recent advances in recombinant DNA technology have paved the way for the production of recombinant proteins that can be used as therapeutic drugs, vaccines and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems (e.g., mammalian cells, bacteria, yeast, insect cells, and transgenic plants) in both laboratory-scale and large-scale environments. The development of efficient bioprocessing strategies is essential for the industrial production of recombinant proteins with therapeutic and prophylactic implications.

Enzyme engineering is a science in which enzymes, enzyme containing organelles or cells (microorganisms, animals, plants) are used in certain reaction devices, using the biocatalytic function of enzymes, and using engineering means to convert corresponding raw materials into useful substances and used in social life. It includes preparation of enzyme preparation, solidification of enzyme, modification and transformation of enzyme and enzyme reactor. Its application is mainly concentrated in the pharmaceutical industry, food industry and light industry.