Yocell Biotechnology (Qingdao) Co., LTD

Carbon nanotubes (CNTs), seamless tubular nanomaterials formed by rolling single or multiple layers of graphene sheets, have caused a sensation in the materials science community since their official discovery by Professor Sumio Ijima in 1991, thanks to their extraordinary mechanical, electrical, thermal and chemical properties. Its huge specific surface area and unique nano-scale hollow structure have enabled it to demonstrate remarkable application potential in numerous cutting-edge fields.

In the biopharmaceutical field, traditional mammalian cell culture has long dominated, but it faces challenges such as high costs and long cycles. Chapter 8 "Production of Complex Proteins in Plants: From Farming to Manufacturing" in Biopharmaceutical Manufacturing: Progress, Trends and Challenges reveals a brand-new "green production pathway" — plant cell culture technology. With its unique advantages, it has emerged as a promising candidate for complex protein production.

Antibody-conjugated drugs (ADCs) are novel tumor treatment drugs that possess both precise targeting and efficient killing capabilities. With advantages such as enhanced efficacy and reduced systemic toxicity, they have become a research hotspot. The tangential flow filtration (TFF) technology is widely used in the downstream process of ADC drugs, capable of efficiently removing organic impurities, coupling related impurities, and achieving buffer solution replacement. The ADC recovery rate exceeds 90%. This technology requires optimizing the process by controlling key parameters such as inlet and outlet pressure, flow rate, and pH. The core application lies in antibody concentration and desalination, product purification, and buffer solution replacement of ADC drugs, providing core support for the efficient preparation and stability of ADC drugs. Yocell's tangential flow system, through technological innovation, helps advance anti-tumor treatment towards precision and reliability.

Monoclonal antibody production relies on the long-term stable culture of mammalian cells such as CHO cells and HEK293 cells, which requires high requirements for sterility, parameter stability and contamination prevention and control of the reactor --- GMP stainless steel reactor adopts 316L medical grade stainless steel tank with mirror polishing and double mechanical sealing.

In the material processing stage of fields such as food, medicine, and bioengineering, "homogenization" is a key process that determines the quality and functionality of the product. It not only makes the particle size of the material more uniform and enhances its stability but also activates the biological activity of the effective components, endowing the product with higher market value. As the core equipment for achieving efficient homogenization, high-pressure homogenizers, with their outstanding technical performance, are becoming the "sharp weapon" for more and more enterprises to break through production bottlenecks and achieve quality upgrades.

Protein, as the material basis of life, is the main carrier of life activities. For downstream processes, new ligand purification technologies are constantly being developed, which can remove various impurities in complex protein samples more safely, economically and efficiently. This paper summarizes the research status, innovative applications and development prospects of medium materials with different ligands (including hydrophobic ligands, ion-exchange ligands, mixed-mode ligands and short peptide biomimetic ligands) and protein separation technologies (including polymer grafting technology and continuous column chromatography technology), with the aim of obtaining effective resources in the protein purification process and providing references for the development of downstream purification technologies.