Isolation of modified protein

Jan 26, 2024

Chromatographic column

NmLOAD 50/600,NW Super 75

Mobile phase

50mM PB+0.1M NaCl (pH 6.8)

Formulation scheme

6.12g NaH2PO4+6.95g NaH2PO4 +11.68g NaCl, adding water to 2L, ultrasonic dissolution filtration

Flow rate


Detecting wavelength


Loadin condition

Quantitative cup sampling, 10mg/ml, injection volume 10ml, 100mg each time

Crude product separation diagram

Target components and crude products

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